人卵巢癌组织差异表达基因的cDNA基因芯片检测(英文)

Detection of differently expressed genes in human ovarian carcinoma by cDNA Microarray

目的探讨人卵巢癌组织差异表达基因,揭示卵巢癌发生、发展的分子机制。方法人卵巢癌组织标本2例,均经病理确诊为低分化浆液性乳头状囊腺癌,临床诊断为卵巢癌Ⅲ期,选用2例正常卵巢组织为对照。提取卵巢组织中mRNA,提取并纯化卵巢癌组织中总RNA,应用cDNA基因芯片技术对总共588个基因进行差异表达基因检测。结果共筛选出差异表达基因44条,其中11条表达上调(包括癌基因c-erbB2、neu、c-fos、c-mycproto-oncogenes、HER2receptor等)、33条表达下调(包括RAR、MMP18、MMP19、p21、DNA-PK等)。结论揭示了人卵巢癌组织中差异表达基因,有望为早期诊断卵巢癌及判断预后提供理想标志物。

Objective To investigate the differently expressed genes in human ovarian carcinoma, and to reveal the molecular mechanism of the cancerous development.Methods The specimens of human ovarian serous adenocarcinoma tissues in stage Ⅲ, and of normal human ovarian tissues as control were excised during surgery for present study. Clinical stages were determined by the Federation International of Gynecology and Obstetrids （FIGO）. Total RNA was isolated from human ovarian tissues, and eDNA probe was labeled and purified The amount of radioactivity incorporated into the cDNA probes was checked by a scintillation counter. The profiles of gene expression were compared between carcinomas and normal ovarian tissues by eDNA mieroarray which contained 588 genes totally. Results Forty four differentially expressed genes were identified from the 588 genes which were from ovarian carcinoma and normal ovarian tissues and compared with eDNA expression array and analyzed by AtlasImage 1.01 software. 11 of the 44 genes were up-regulated in ovarian carcinoma tissues （including c-erbB2, neu, c-los, c-myc proto-oncogenes, HER2 receptor, and so on）, and the other 33 genes were down-regulated （including RAR, MMP18, MMP19, p21, DNA-PK, and so on）. Conclusion The gene expressions in human ovarian carcinoma have been detected in present study. It is the differently expressed genes that help us to disclose the potential molecular mechanisms of the developmental process of human ovarian carcinogenesis. The differendy expressed genes may provide a useful hallmark for the early diagnosis of human ovarian carcinoma.