大鼠视皮层神经元原代培养

Primary culture of visual cortical neurons of the newborn rat

目的通过原代培养新生大鼠视皮层神经元,探求视皮层神经元的最佳分离和培养方法,提高原代培养神经元的纯度及活性,为视皮层相关性眼病的临床基础研究提供实验模型。方法分离新生大鼠视皮层神经元,应用含10%新生牛血清、10%F-12 Nutrient Mixtures的DMEM培养基进行接种培养,含2%B-27 Serum-Free Supplements的Neu-robasal Medium进行维持培养,利用尼氏染色进行神经元鉴定。结果培养的神经元生长良好,胞体饱满,突起长。尼氏染色示神经元比例大于50%。结论采用上述培养方法可以使新生大鼠视皮层神经元得到良好的生长和较高的纯度。

Objective To establish an experimental model in the primary culture of visual cortical neurons of the newborn rat for the study of ophthalmic diseases related to visual cortical neurons. Methods Primary culture of visual cortical neurons of the newborn rat was established in Dulbecco＇s modified Eagle＇s medium with 10% newborn calf serum and 10% F-12 Nutrient Mixtures and Neurobasal Medium with 2% B-27 Sernm-Free Supplements. Evaluation of neurons was done with Nissl＇s staining. Results Cultured neurons grew well with satiety and long apophysis. The purity of the neurons was higher than 60%. Conclusion Primary culture of visual cortical neurons of the newborn rat is a reliable method for obtaining active neurons with high purity.