温度和pH对白斑狗鱼消化酶活性的影响

Effects of Different Temperature and pH on Digestive Enzyme Activities in Esox lucius

目的:通过改变酶的反应温度和pH,离体分析白斑狗鱼体内淀粉酶、蛋白酶和脂肪酶活性变化。方法:分别采用Folin酚法、DNS法和氢氧化钠滴定法测定蛋白酶、淀粉酶和脂肪酶活性。结果:在白斑狗鱼肝胰脏、胃二部位,淀粉酶的最适温度均为30℃,肠道淀粉酶的最适温度为40℃;最适pH值分别为4.0、2.0、7.0。肝胰脏、胃二部位脂肪酶的最适温度均为40℃,肠道脂肪酶的最适温度为50℃;最适pH值分别为5.0、4.0、5.0。肝胰脏、胃、肠道蛋白酶的最适温度均为50℃;最适pH值分别3.0、3.0、9.0。结论:在各自最适温度下,脂肪酶、淀粉酶、蛋白酶比活力均为:肠道>胃>肝胰脏。

Obtective： By changing temperature and pH, the Amylase, Protease and Lipase of Esox lucius were studied in vitro. Methods： The activities of enzymes were analyzed by Folin - Phenol, DNS, NaOH titration separately. Results： The results shown that the optimum temp. of Amylase were 30℃ from hepatopanereas and stomach, whereas was 40℃ from intestine. And their relevantly optimura pH were 4.0, 2.0 and 7. 0, respectively. The optimum temp. of Lipase were 40℃ from hepatopancreas and stomach, whereas was 50℃ from intestine. And their optimum pH were 5.0, 4.0 and 5.0, respectively. The optimum temp. of Protease from three digest organs were all 50℃, while their optimum pH were 3.0, 3.0 and 9.0, respectively. Conclusion： At their optimum temprature point, the Rate activity of Amylase was the highest than those of Lipase and Protease in digest organ of Esox Iucius.