1-(4-安替比林偶氮)-2-羟基-4-二甲苯胺的合成及其与铜(Ⅱ)显色反应的研究

Synthesis of 1-(4-antipyrinylazo)-2-hydroxyl-4-dimethylaniline(APHMA) and study on its color reaction with copper(Ⅱ)

合成了新显色剂1-（4-安替比林偶氮）-2-羟基-4-二甲苯胺（APHMA），并研究了其与Cu（Ⅱ）的显色反应。在pH4．0的HAc-NaAc缓冲介质中，APHMA与Cu（Ⅱ）生成2：1稳定络合物，最大吸收波长为560nm，摩尔吸光系数为2．36×10^4L·mol^-1·cm^-1，Cu量在0～1．0μg／mL内符合比耳定律。已用于食品中铜的分析。

In this paper, a new chromogenic reagent 1-（4-antipyrinylazo）-2-hydroxyl-4-dimethylaniline （APHMA） was synthesized and the color reaction of APHMA with copper（ Ⅱ ） was studied. Results showed that a red complex of APHMA and Cu（ Ⅱ ） was formed in a buffer solution of HAc-NaAc at pH 4 in the presence of Tween-80, which gave a maximum absorption at 560 nm with an apparent molar absorptivity of 2.36 × 10^4 L·mol·cm^-1, Beer＇ s law was obeyed in the range of 0 - 1.0μg/mL for determination of copper.