青海栽培何首乌不同部位中蒽醌类成分的测定

Analysis of anthraquinones in polygonum multiflorum by RP-HPLC

用高效液相色谱法测定青海栽培何首乌中的主要有效成分大黄素、大黄素甲醚等蒽醌类成分。采用kromasil C18柱（4．6mm i．d．×250mm，5μm）；V（甲醇）：V（水）：V（H3PO4）=750：250：0．001为流动相，检测波长为254nm，流速为1mL／min，进样量10μL。用此方法测定青海栽培何首乌不同部位中的蒽醌类成分，大黄素和大黄素甲醚达到基线分离，线性范围分别为0．094～1．50／μg（r=0．9992），0．094～1．50／μg（r=0．9997），回收率分别为大黄素95％、大黄素甲醚102％。实验发现青海栽培何首乌块根中大黄素和大黄素甲醚成分较藤、叶中高，大黄素含量比大黄素甲醚含量高。

High performance liquid chromatergraphic method was developed for the determination of emodin and physcion in polygonum multiflorum. A phenomenex kromasil C18（4.6 mm × 250 mm, 5 μm） column was used, methanol-water-phosphate acid （750：250：0.001） was used as the mobile phase. UV detection wavelength was 254 nm, and the flow rate was 1 mL/min. This method was applied to determining anthrequinones in different parts of polygonum multiflorum, two detected compounds were isolated on base line. The linear ranges of emodin and physcion were 0.094 - 1.50 μg （ r = 0.9992）, 0.094 - 1.50μg （ r = 0.9997）. The average recoveries were 95%, 102%. The results showed that the contents of emodin and physcion in the mot were higher than the others, and the content of emodin was higher than that of physcion in the mot of polygonum multiflomm cultivated in Qinghai.