一株苯酚降解菌的分离及降解特性

Isolation and degradation characteristics of a phenol degrading bacteria

利用以苯酚为唯一碳源和能源的无机盐溶液作为驯化液,对某废水处理厂活性污泥进行驯化培养,从中分离筛选到1株苯酚降解菌,编号为BW-1。该菌株最高可耐受2000mg/L的苯酚。对该苯酚降解菌降解性能研究表明:该菌具有较强的苯酚降解能力,在35℃、pH值为6.0～7.5、装液量为60mL、接种量20%、摇床振荡速度120r/min的条件下,反应6h后可使400mg/L的苯酚降解率达80%以上;葡萄糖对菌体的生长及苯酚降解能力均有一定的影响;当葡萄糖浓度是600mg/L时,该菌对苯酚的降解率仍在60%以上。该研究对处理含有其它碳源的含酚废水具有一定的意义。

A kind of phenol degrading bacteria （BW-1）, which was able to grow on phenol as sole carbon and energy sources, was isolated from the activated sludge of Northern Shenyang Wastewater Treatment Plant. The highest endurance capability of this bacterial strain for phenol was 2000 mg/L. The results showed that the bacterial strain had strong phenol degradation capability. The phenol degradation rate was above 80% when phenol concentration was 400 mg/L, the temperature was 35℃, the pH value was 6.0-7.5, the start culture medium was 60mL, the inoculation volume was 20%, the speed of shake bed was 120 r/min and reaction time was 6 hours. Gucose had some effects on the growth of bacteria and phenol degradation capability. When glucose condentration was 600 mg/L, phenol degradation rate of the bacterial BW-1 was still above 60%. It could be useful for treating phenol-containing wastewater with other kinds of carbon sources.