摘要
采用HPLC法测定复方天然药物一温必佳注射液中黄芩苷的含量。色谱柱ZORBAXSB—C18柱(150mm×4.6mm,5μm),流动相为甲醇-水-冰醋酸(50:50:1),检测波长274nm,流速1.0mL/min,柱温30℃,保留时间为5.5min。黄芩苷进样量在0.1032—2.064μg范围内,峰面积与进样量之间呈良好的线性关系(R^2=0.99997)。平均回收率99.3%,RSD为0.78%。该方法适用于检测中药新制剂——温必佳注射液中黄芩苷的含量。
An HPLC method has been developed to determine the baicalin in Wenbijia injection (a complex prescription of naturally occurring drugs), using ZORBAX SB - C18 column( 150 mm ×4.6 ram,5μm) with a mobile phase of methanol -water- acetic acid glacial(50: 50:1 )at the wavelength of 274 nm . The flow rate was 1.0 mL/min, column temperature was kept at 30 ℃, and the retention time was 5.5 min. The linear relationship of baicalin was good in range of 0. 103 2 - 2.064 p.g ( R2 = 0.999 97 ). The average recovery was 99.3% with RSD of 0.78%. This method can be used to control the quality of baicalin in Wenbijia injection.
出处
《中国兽药杂志》
2008年第2期42-44,共3页
Chinese Journal of Veterinary Drug