α7-烟碱样乙酰胆碱受体基因多态性与精神分裂症的关联研究

The association between schizophrenia and the nicotine-like acetylcholinergic receptor gene polymorphism

目的研究α7-烟碱样乙酰胆碱受体基因rs1042724多态性与精神分裂症的相关性。方法运用聚合酶链反应扩增及单核苷酸多态性的分子生物学技术,对符合精神分裂症诊断标准的98个先证者及其父母组成的核心家系,测定α7-烟碱样乙酰胆碱受体基因分型,进行精神分裂症的α7-烟碱样乙酰胆碱受体基因多态性的关联分析和传递不平衡(TDT)检验。结果TDT检验结果提示α7-烟碱样乙酰胆碱受体基因等位基因与精神分裂症之间的相关性(M cNem ar2χ=4.21,P<0.05),且等位基因T携带者,其精神分裂症的易患性是C携带者的1.31倍(RR=1.31,2χ(RR)=3.96,P<0.05)。结论提示α7-烟碱样乙酰胆碱受体基因rs1042724与精神分裂症相关联。

Objective To detect the genetic association between schizophrenia and rs1042724 polymorphism at nueleotide of the nicotine - like acetylcholinergic receptor gene. Methods Observed in a sample of 98 parent/offspring trios where the proband net the amerecan classification and diagnostic criteria for mental disorders. The forth revised edition, criteria for schizophrenia using transmission/disequilibrium test（TDT） analysis. The polymorphism of nicotine -like aeetylcholinergic receptor gene was detected with PCR methods and SNP typing in all nucleus families. Results Using a family - based association design we obtained evidence of an association between schizophrenia and allele of the rs1042724 polymorphism in the nicotine - like acetylcholinergic receptor gene （ RR = 1.31 ,X^2 （RR） = 3.96, P 〈 0. 05 ）. Moreover the fact that transmission disequilibrium was observed when one affected offspring was selected at random from each family, suggesting that the results are due to association（ McNemarX^2 = 4. 21 ,P 〈 0. 05 ）. Condttsion It shows an association between schizophrenia and the rs1042724 polymorphism at nucleotide of the nicotine - like aeetylcholinergic receptor gene in Chinese.