阿尔茨海默病大鼠模型PET在体成像研究

The Use of PET Imaging in the Evaluation of Alzheimer's Disease Rat Model

目的研究18F-FDG及Aβ蛋白显像剂(4’-schiff-O[11CH3])PET成像技术在判别阿尔茨海默病大鼠模型中的作用。方法对10只注射Aβ1～40复制阿尔茨海默模型大鼠及10只注射生理盐水对照大鼠行HE染色及刚果红染色,检测大鼠脑内病理学改变。用18F-FDG及4’-schiff-O[11CH3]PET显像技术在体观察2组大鼠脑内Aβ蛋白分布及葡萄糖代谢情况。结果模型组大鼠海马出现神经元减少并形成淀粉样斑块。模型组PET显像可见注射侧海马葡萄糖代谢减低及4’-schiff-O[11CH3]摄取增加。对照组仅见轻微神经元损伤,PET显示葡萄糖代谢轻度减低。结论18F-FDG及4’-schiff-O[11CH3]PET显像结合行为学及病理学观察可作为检测模型成功与否的方法之一。

Objective To investigate the value of ^18F-FDG and 4＇ -schiff-O [ ^11CH3 ] PET imaging in the evaluation of Alzheimer＇ s disease rat model. Methods Ten AD rat models and 10 control rats had undergone the ISF-FDG and 4＇ -schiff-O [ ^11CH3 ] PET studies. The sections of the brain tissues of both control and AD model rats were stained with HE and Congo red. Results PET imaging of AD rats showed reduced glucose metabolism and high uptake of 4＇-schiff-O [ ^11CH3 ] in the injected side. Diffuse neuronal loss and Aβdeposition were detected in the hippocampi and cortices in the AD rats ; in contrast, such changes were almost not seen in control rats. Conclusion ^18F-FDG and 4＇-schiff-O[ ^1CH3 ] PET imaging , combining with the behavioral and pathological observations can set up standards of verifying the AD rat model, and it provides a molecular imaging tool for the basic research and clinic diagnosis of AD.