胶质瘤细胞BT325和U251凋亡相关因子的表达差异

Comparison between the Expressions of Apoptosis-related Factors of BT325 Human Glioblastoma and U251 Glioblastoma

目的比较BT325和U2512种胶质瘤细胞中凋亡相关因子的表达差异及其在凋亡中的作用。方法应用Western blot方法检测细胞中Fas、Caspase-3、Caspase-7、Survivin和Livin的表达情况。结果U251与BT325均有Fas、Caspase-3和Livin蛋白表达,BT325的Caspase-3含量高于U251,U251的Livin含量高于BT325;Survivin仅表达于U251;Caspase-7仅表达于BT325。结论BT325胶质瘤细胞中凋亡效应因子呈高表达,U251胶质瘤细胞中凋亡抑制因子呈高表达。

Objective In previous study, we have confirmed that BT325 glioblastoma underwent more severe apoptosis than U251 glioblastoma under the same apotosis inductive conditions. In order to find some explication, we planned to extract the total protein from BT325 and U251 glioblastoma cells cultured in vitro, and investigate the expression of apoptosis-related factors of these glioblastomas. Methotls Fas, caspase-3, caspase-7, survivin and livin expressed by BT325 and U251 glioblastomas were investigated by Western blot. Results There were no significant difference between the expression levels of Fas of BT325 glioblastoma and U251 glioblastoma. Both caspase-3 and caspase-7 were expressed in BI325 glioblastoma, and only caspase-3 expressed in U251 glioblastoma. In addition, the expression level of caspase-3 in U251 glioblastoma was lower than that of BT325 glioblastoma. Livin is a new family member of IAP, which was discovered recently. It can combined with the activated executive factors of the downstream apoptosis signaling cascades such as easpase-3 and caspase-7 and restraining their activity, so inhibit apoptosis. Survivin is a family member of IAP with the minimal molecular weight, and also it is the strongest inhibit factor of apoptosis discovered up to now. In the test, we found survivin and livin were expressed significandy in U251 glioblastoma simultaneously, whereas only livin was expressed in BT325 glioblastoma, besides, its expression level was lower than that in U251 glioblastoma. Conclusion Apoptotic effector expressed significantly in BT325 glioblastoma and apoptotic inhibitor expressed significantly in U251.