同仁安神丸主要药效学实验研究

Studies on the Main Pharmacological Effects of Tongren'anshen Pill in vivo

目的对同仁安神丸主要治疗作用进行药效学研究。方法灌胃给予同仁安神丸原粉(不含蜜),观察其对小鼠抗尼可刹米诱发惊厥发作的作用,对睡眠、学习记忆、自主活动及对小鼠失血性贫血作用的影响。结果同仁安神丸能明显延长小鼠抗尼可刹米诱发惊厥发作潜伏期(P<0.05);能延长小鼠死亡时间,但无统计学意义;可明显延长小鼠服用戊巴比妥钠后睡眠时间(P<0.05),缩短入睡潜伏期(P<0.05);明显增加戊巴比妥钠阈下剂量小鼠入睡只数(P<0.003);明显减少小鼠的自主活动次数(P<0.05);明显增加失血小鼠血红蛋白(Hb)含量(P<0.05);但对小鼠学习记忆行为无明显影响。结论同仁安神丸具有镇静安神养血的作用,与传统临床应用效果一致。

Objective To investigate the main pharmacological effects of Tongren＇ anshen Pill in vivo. Methods The mixture of the powdered ingredients（ without honey） was administered peros to mice and the effects on independent activity, memory, sleeping and the prevention of nikethamide-induced convulsion and hemorrhagic anemia were observed. Results After administration of the mixture, the independent motion number of times was obviously reduced （ P 〈 0. 05 ）, but the memory was not influenced. For the nikethamide- induced convulsion, the incubation period was obviously prolonged （ P 〈 0.05 ）. The survival time was prolonged, but not significant statistically. For the pentobarbital-induced sleeping, the sleeping-time was obviously prolonged （ P 〈 0.05 ）. The incubation period was shortened significantly（ P 〈 0.05 ） and the number of mice falling a sleep under the threshold dose of pentobarbital was obviously increased （P 〈0.05 ）. The Hb content in hemorrhagic mice was obviously increased （P 〈 0.05 ）. Conclusion Tongren＇ anshen Pill can tranquilize the mind and nourish the blood which are consistent with its clinical application.