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RNA干扰及其在肿瘤研究中的应用 被引量：2

RNA Interference in Cancer Research
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摘要 RNA干扰(RNAi)是近年发展起来的一种阻抑基因表达的新方法,该技术通过双链RNA的介导,可以特异性地阻断或降低相应基因的表达。在肿瘤研究中,通过RNAi技术可以选择性地抑制人类肿瘤相关基因的表达,从而抑制肿瘤细胞的生长。该技术的应用为癌症的基因治疗提供了新的方法。本文综述了小干扰RNA(siRNA)、微小RNA(miRNA)的生成和作用机制以及RNAi在肿瘤研究中的应用。 RNA interference （RNAi） is a newly developed method to negatively regulate gene expression by introduction of homologous double stranded RNA（dsRNA） to specifically act on a gene＇s product, resulting in the formation of null or hypemorphic phenotypes. Discovered only recently, it has been utilized very successfully to inhibit many tumor cells growth by knocking down some oncogenes expression, suggesting an enthusiastic potential for therapeutic applications. In this review, it summarizes recent views about the mechanism of small interference RNA（siRNA） and microRNA production and process, and the current and future potential in cancer diagnosis and prevention.
作者 程杉 熊英 贺俊崎
机构地区 首都医科大学生物化学与分子生物学系
出处 《首都医科大学学报》 CAS 2008年第1期87-95,共9页 Journal of Capital Medical University
基金 国家自然科学基金资助项目(30371643 30572183) 北京市科技新星计划(2007B065) 首都医科大学基础临床合作(2007JL01)~~
关键词 RNA干扰 小干扰RNA 基因沉默 肿瘤 RNA interference small interfering RNA gene silencing tumor
分类号 Q78 [生物学—分子生物学]
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共引文献29

同被引文献17

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首都医科大学学报
2008年 第1期

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