肝病患者Ⅰ型前胶原羧基末端肽与多项肝硬化指标联合检测的分析

Co-measurements of PICP with Col Ⅲ,LN and AFP in the Diagnosis of Liver Disease

目的探讨Ⅰ型前胶原羧基末端肽(PICP)在肝病中的诊断意义及其与Ⅲ型胶原(ColⅢ)、层粘连蛋白(LN)和甲胎蛋白(AFP)的关系。方法采用酶联免疫吸附试验和化学发光法测量乙型肝炎、肝硬化、肝癌患者和健康者(对照组)血清PICP、ColⅢ、LN和AFP的水平。结果各肝病组患者血清PICP、ColⅢ、LN和AFP水平与对照组比较,差别均有统计学意义(P<0.01),不同肝病组之间上述指标比较,差别也具有统计学意义(P<0.05)。各肝病组患者血清PICP水平与LN水平呈正相关(r=0.997,P<0.01)。结论肝病患者血清PICP水平明显升高,尤其是肝癌患者;血清PICP水平与LN水平呈正相关。

Objective To investigate the significance of measuring C - terminal propeptide of type Ⅰ. procollagen （PICP） in diagnosing liver disease and its relationship with type Ⅲ collagen （Col Ⅲ） , laminin （LN） and Alpha- fetoproteins （AFP）. Methods The enzyme linked immunosorhent assay （ELISA） and Chemiluminescence were taken to assay the concentration of PICP, Col Ⅲ, LN and AFP in serum of the patients with hepatitis, hepatocirrhosis and liver cancer with the health persons as the controls. Results In the liver disease patients, the values of serum PICP, Col Ⅲ, LN were more high then normal controls （ P 〈0. 01 ）. The levels of PICP, Col Ⅲ, LN and AFP were also significantly different among various liver disease groups （ P 〈 0. 05 ）. The level of serum PICP was positively correlated with LN （ r = 0. 997, P 〈 0. 01 ）. Conclusion The level of serum PICP is highly increased in patients with liver disease, especially in those with liver cancer. There is a positive correlation between the levels of PICP and LN.