重症急性胰腺炎大鼠肿瘤坏死因子产生及吸收部位的研究

Experimental research on the site of production and uptake of TNF in rats with severe acute pancreatitis

目的:探索重症急性胰腺炎(SAP)大鼠肿瘤坏死因子(TNF)产生及吸收的部位,以及TNF与内毒素(CET)之间的关系.方法:采用逆行加压注入5%牛磺脱氧胆酸钠溶液复制SAP,采取门静脉、肝静脉及股动脉血,检测其血清TNF含量和门静脉血浆ET含量.结果:在SAP发展过程中门静脉血清TNF含量迅速升高;在6小时时肝静脉、股动脉血清TNF含量低于门静脉血清TNF含量(P<0.05);但股动脉血清TNF含量高于肝静脉血清TNF含量(P<0.05).门静脉血浆ET含量的升高明显迟于血清TNF含量的升高.结论:在SAP的恶性发展过程中,胰腺、肠道、脾脏及肺均是产生血清TNF的重要器官;肝脏是清除TNF的主要场所,血浆ET并不是血清TNF产生的始动因素.

The site of production and uptake of tumor necrosis factor (TNF) in rats with severe acute pancreatitis (SAP) and the relationship between TNF and endotoxin (ET) were studied. Sprauge Dawley rats were randomly devided into control group and SAP group. SAP model was shaped by retrograde injection of 5% sodium taurodeoxideocholate (1.5ml/kg) via pancreatic bile duct. The blood samples were obtained from portal vein , hepatic vein and femoral artery 2 h and 6 h after having made the model in order to assay the amount of TNF in serum and the amount of ET in the plasma from portal vein. The results revealed that the content of TNF in portal vein increased rapidly in the development process of SAP. The contents of serum TNF were lower in hepatic vein and in femoral artery than that in portal vein (P<0.05). The contents of serum TNF were higher in femoral artery than that in hepatic vein (P<0. 005). The increase of plasma ET was later than serum TNF in portal vein. Therefore, pancreas, intestinal tract, spleen and lung are the main organs to produce TNF, while, liver is an important site for scanvenging TNF in the vicious development process of SAP. ET is not an incipient factor to stimulate the releasing of TNF in rats with SAP.