摘要
对金鱼（Carassius auratus）核糖体转录间隔区（ITS-1）进行了克隆测序，并对ITS-1序列进行了分析。结果显示，克隆的金鱼ITS-1区序列长度为294bp，其中A、T、G、C4种碱基的含量分别为17．0％、14．6％、33．0％、35．4％；与从GenBank中检索到的12种鱼的核糖体DNA序列比较显示，其与12种鱼的ITS-1序列同源性较低，在26．0％～61．6％之间；根据金鱼与其他12种鱼的ITS-1序列的同源性构建进化树，所得的分类结果与传统的分类结果基本一致。
The internal transcribed spacer 1 （ IST-1 ） from goldfish （ Carassius auratus ） was cloned, sequenced and analyzed. The result showed a sequence with total length of 294 nucleotides of ITS-1. The contents of A, T, G and C were 17. 0% , 14.6% , 33.0% , 35.4% in ITS-1 of goldfish. Comparing to ITS-1 sequences of other 12 fishes in GenBank, there were lower homologies from 26.0% to 61.6% between goldfish and other fishes. The evolution tree built from the ITS-1 homology of goldfish and other 12 fishes was consistent with that of traditional taxonomic results.
出处
《淡水渔业》
CSCD
北大核心
2008年第1期74-76,43,共4页
Freshwater Fisheries
基金
广东省农业攻关计划项目(2005B20301015)
