镧铈对癌细胞恶性增殖及其相关基因表达的影响

Effects of Lanthanum and Cerium on Malignant Proliferation and Expression of Tumor related Gene

为进一步证实稀土元素的抗肿瘤作用，并探讨其抑癌作用机理，以体外培养的人胃癌细胞ＰＡＭＣ８２为研究对象，观察镧、铈元素对癌细胞生长的抑制作用，降低恶性程度的逆转作用及对细胞中癌基因、抑癌基因表达水平的影响。氯化镧、氯化铈和混合氯化稀土在０．５～１．５ｍｍｏｌ／Ｌ浓度下可显著抑制癌细胞生长。并使ＰＡＭＣ８２细胞形态、微管结构发生类似正常细胞的转变。在软琼脂中的生长能力下降。抑癌基因ｐ５３、ｐ１６和ｐ２１表达水平上升，ｎｍ２３基因表达水平下降。

Cultured human gastric cancer cell line PAMC82 was studied in vitro to further verify anti tumor effects of rare earth elements and explore their mechanism of tumor inhibition. Inhibitory effects of elements lanthanum and cerium on cell growth, reverse effects of them on reduction of malignancy and effects of them on level of expression of oncogene and cancer suppressor gene were observed. Lanthanum chloride, cerium chloride and mixed rare earth chloride at levels of 0.5 to 1.5 mmol/L could inhibit obviously growth of cancer cells and change cell morphology and microtubule structure of PAMC82, similar to that of normal cells, their colony forming ability lowered in soft agar, and expression of tumor suppressor gene p53, p16 and p21 increased and that of gene nm23 lowered.