某市自来水有机提取物诱导的人胚肺细胞恶性转化实验研究

Studies on Malignant Transformation of Human Fetal Lung Cells Induced by Organic Extract from Tap Water

以Ｇ市Ｚ江的自来水有机提取物为样品，在诱导人胚肺细胞恶性转化基础上，于转化过程中及结束时分别测定了Ｐ５３蛋白的表达情况，以探讨恶性转化的分子机理，结果表明：水样在实验剂量范围内可不同程度地诱导细胞恶性转化灶的形成，并有一定的细胞毒性；染毒１５天细胞还未形成转化灶前和染毒３０天已形成转化灶后细胞核中均出现了Ｐ５３蛋白的高表达。提示：Ｐ５３蛋白的改变很可能与细胞的恶性转化有一定的关系，并在细胞恶性转化过程中起一定的作用。

Expression of P53 protein was determined in the process and the end of induction of malignant transformation in human fetal lung cells, with organic extract from tap water in a river of City G, to study its molecular mechanism. Results showed that the water sample of experimental doses could induce formation of cell malignant transformation foci to different extent and had certain toxicity to cells. High expression of P53 protein was observed in cell nuclei 15 days after exposure and before formation of cell transformation foci, or 30 days after exposure and formation of them. It suggests that changes of P53 protein may relate to cell malignant transformation to certain extent, and play some roles in the process of it.