反相高效液相色谱法测定十味诃子散中的没食子酸

Determination of Gallic Acid in Shiwei Hezi Powder by RP-HPLC

采用反相高效液相色谱法，用十八烷基硅烷键合硅胶为填充剂，甲醇-水（V甲醇：V水，5：95）为流动相，检测274nm处波长。结果表明：十味诃子散中没食籽酸含量的线性范围为0．0206～0．2472μg，平均回收率99．6％，RSD=1．6％（n=9）。

To establish a quantitative method to determine the content of gallic acid in Shiwei Hezi powder. The content of gallic acid in Shiweihezi powder was determined by RP-HPLC. The separation was performed on Kromasil Ciscolumn with a mobile phase of methanol-water （5：95） and detected at wavelength of 274 nm. Results showed that the linearity range of the gallic acid content in gallic acid in Shiwei Hezi powder was 0. 020 6 -0. 247 2 μg and the average recovery of gallic acid was 99.6% , RSD was 1.6%.