摘要
[目的]观察黄芪(AM)对单侧输尿管梗阻(UUO)所致大鼠肾小管间质纤维化的影响,初步探讨其抗纤维化分子作用机制。[方法]48只SD大鼠随机分为假手术(Sham)、UUO组和UUO+黄芪治疗组(AM)、UUO+氯沙坦治疗组(ARB)。在造模后7、14d处死动物,观察肾脏病理改变。采用免疫组化方法检测肾组织中纤连蛋白(FN)和胶原Ⅰ(colⅠ)表达。用实时荧光定量RT-PCR检测大鼠FNmRNA和colⅠmRNA表达量。[结果]与假手术组相比,UUO术后大鼠肾脏病理损害进行性加重,FN和colⅠ表达显著增高(P﹤0.05);AM治疗后可明显减轻UUO大鼠肾组织的肾小管损伤及肾间质纤维化,使UUO大鼠肾组织中高表达的FN和colⅠ明显降低(P﹤0.05),与ARB作用相似(P﹥0.05)。[结论]黄芪可能通过抑制肾小管间质细胞外基质积聚,减轻和改善UUO大鼠肾间质纤维化。
[Objective] To study the effect of Astragalus mongholicus (AM) on renal fibrosis in SD rats with Unilateral Ureteral Obstruction (UUO), and to explore the molecular mechanisms of AM of anti-fibrosis. [Methods] 48 Sprague-Dawley rats were randomly divided into 4 groups: sham-operation group (Sham), the UUO group (UUO), UUO + AM group(AM) and UUO + Losartan group (ARB). At 7, 14 days after UUO, the dynamic histological changes of renal interstitial tissues were observed. The expression of fibronectin (FN) and collagen I (col I ) were measured by immunohistochemistry staining sections. The mRNA of FN and col I were reversed transcribed and quantified by real-time PCR. [Results] Renal damage was exacerbated and the expression of FN and col I were all significantly increased in UUO group compared with those of Sham group (P 〈 0.05) at each time point. Tubular lesions and interstitial fibrosis were alleviated, and up-regulations of expressions of FN and col I were significantly suppressed by AM treatment (P 〈 0.05), and there were no significant difference between AM and ARB groups (P 〉 0.05). [Conclusion] AM can ameliorate renal interstitial fibrosis induced by UUO in rats. The mechanisms of its antifibrosis effects may be related with the suppression of the accumulation of extra cellular matrix.
出处
《现代预防医学》
CAS
北大核心
2008年第4期784-787,共4页
Modern Preventive Medicine
关键词
黄芪
单侧输尿管梗阻
细胞外基质
肾间质纤维化
Astragalus mongholicus
Unilateral ureteral obstruction
Extra cellular matrix
Renal interstitial fibrosis