摘要
目的研究Wnt通路在胃癌组织中的活化状态及其与胃癌发生发展的关系。方法应用免疫组化、免疫荧光、RT-PCR和Western蛋白印迹等方法,在胃癌组织和细胞系中检测Wnt信号途径的活化状态。结果Wnt 2、β-catenin和TCF4在胃癌组织中的表达明显高于癌前病变组织(P<0.01);Wnt组与Wnt-组中癌前组织和胃癌组织的分布具有显著统计学差异(P<0.001)。结论Wnt信号通路在胃癌组织中呈活化状态,并与胃癌发生发展密切相关。
Objective To illustrate the activation status of Wnt signaling and its correlation with gastric carcinogenesis. Methods Immunohistochemistry, immunofluorescence, RT-PCR and western blotting were adopted to detect Wnt signaling activation in the gastric cancer tissues and cell lines. Results Expressions of Wnt2, β-catenin and TCF4 were significantly higher in the cancer tissues, compared with premalignant tissues （ P 〈 0.01 ）. The distributions of Wnt ^＋ and Wnt^ - groups were significantly different in premalignant and cancerous tissues （ P 〈 0. 001 ）. Conclusion Wnt signaling is not only activated in gastric cancer tissues but associated with gastric carcinogenesis as well.
出处
《临床军医杂志》
CAS
2008年第1期7-9,共3页
Clinical Journal of Medical Officers
基金
国家自然科学基金资助项目(30670946)
