人胃癌、肝癌的c-myc癌基因甲基化的研究

Patterns of c-myc oncogene methylation in human gastric and hepatocellular cancer

目的：了解人胃癌和肝癌相关癌基因的甲基化情况。材料与方法：将22例进展期胃癌的癌区、癌旁和外周正常区组织和33例原发性肝癌的癌区、癌旁区及10例正常肝组织的DNA，以限制性内切酶Hpall／Mspl消化、Southernblot分析其c－myc癌基因片段的甲基化情况。结果：发现47％（10／22）的胃癌区、59％（13／22）的胃癌旁和30％（10／33）的肝癌区、13％（4／33）的肝癌旁组织的c－myc癌基因呈低甲基化状态。结论：在消化系常见的恶性肿瘤的发生中，某些癌基因甲基化水平降低。

Objective: To observe the patterns of c-myc oncogene methylation in advanced human gastric and hepatocellular cancer. Methods: The methylation status of c-myc oncogene fragments containing CCGG sequence were digested with endonucleases Hpall/Mspl and analysed by Southern blot in samples from cancerous, paracancerous and non-cancerous tissues in 22 cases of advanced human gastric cancer, and cancerous, paracanerous tissues in 33 cases of hepatocellular carcinoma. Results: The results indicate that c-myc oncogene fragment containing CCGG sequence is hypomethylated of DNA in samples of gastric cancer (47%), paracancerous tissue (59%), and in hepatocellular carcinoma (30%), as wellas its paracancerous tissue (13%).Conclusion:The levels of c-myc oncogene methylation are reduced in human gastric and hepatocellular carcinogenesis.