肥大细胞及其活化状态在克隆病发病中的作用

Effect of mast cells and their activated state in pathogenesis of Crohn's disease

目的：通过观察肥大细胞的活化状态与克隆病病变的关系，探讨肥大细胞在克隆病发病中的作用。方法：采用甲苯胺蓝染色和免疫组化IgE染色对27例克隆病手术切除标本中活化肥大细胞的密度和分布特征进行观察，并对病变活动区和非活动区中该细胞的密度加以比较。结果：克隆病病变组织中可见多量活化肥大细胞，尤以粘膜和粘膜下层为著，并与小血管或神经纤维有密切关系。病变活动区中的活化肥大细胞密度显著高于非活动区和对照组（P＜0.01）。结论：活化的肥大细胞可能在克隆病的发病过程中起重要作用。

Objective: To determine the effect of mast cells on the pathogenesis of Crohn's disease by observation of relationship between the activated state of mast cells and lesion of Crohn's disease. Methods: The density and characteristic of distribution of the activated mast cells in tissue specimens of enterocolectomy from 27 cases of Crohn's disease were observed by using toluide blue staining and immunohistochemical staining for IgE. The density of the activated mast cells in active region was compared with that in non-active region. Results: Numerous activated mast cells ware found in the intestinal lesions of Crohn's disease, especially in the mucosa and submucosa. A close relationship between activated mast cells and small blood vessels or neurofibrils was shown. The density of activated mast cells in active lesions was significantlly higher than that in non-active lesions and in controls (P<0.01). Conclusion: The results suggest that the activated mast cells may play an important role in the pathogenesis of Crohn's disease.