摘要
The prion protein (PrP) encoding gene of flounder (Paralichthys olivaceus) was cloned. It was not interrupted by an intron. This gene has two promoters in its 5' upstream, indicating that its transcription may be intensive, and should have an important function. It was expressed in all 14 tissues tested, demonstrating that it is a house-keeping gene. Its expression in digestion and reproduction systems implies that the possible prions of fish may transfer horizontally.
The prion protein （PrP） encoding gene of flounder （Paralichthys olivaceus） was cloned. It was not interrupted by an intron. This gene has two promoters in its 5＇ upstream, indicating that its transcription may be intensive, and should have an important function. It was expressed in all 14 tissues tested, demonstrating that it is a house-keeping gene. Its expression in digestion and reproduction systems implies that the possible prions of fish may transfer horizontally.
基金
Supported by the Project under Major State Basic Research of China (No. G199901205)
关键词
比目鱼
蛋白质编码基因
基因表达
基因克隆
Bastard halibut
Paralichthys olivaceus
prion protein
PrP
gene
clone
expression
