超高效液相色谱法同时测定维生素强化食品中多种水溶性维生素

The Simultaneous Determination of Water-soluble Vitamins in Fortified Foods by Ultra Performance Liquid Chromatography

目的建立可同时测定维生素强化食品中7种水溶性维生素的超高效液相色谱法。方法采用梯度洗脱超高效液相色谱法，Waters ACQUITY UPLC^TM BEH C18柱（2．1mm×50mm，1．7μm），流动相A为辛烷磺酸钠0．8g／L水溶液（冰乙酸调pH=2．8）；B为甲醇，检测波长270nm，流速0．4ml／min。结果各种维生素含量测定方法的线性关系良好，r=0．9992—0．9998，回收率98．1％～100．2％，RSD=0．17—0．72。用该法测定了市售维生素饮料和维生素强化食品中维生素的含量，结果准确。结论本方法操作简便，分析速度快，灵敏度高，精密度好，结果准确可靠。

Objective To establish ultra performance liquid chromatography（UPLC） method for the analysis of vitamin B1 ,B2, B6, C,folic acid, nicotinic acid and nicotinamide. Methods UPLC was adapted on Waters ACQUITY UPLC^TM BEH C18 column（2. 1 mm ×50 mm,1.7 μm） ,the mobile phase consisted of A：0. 8 g/L 1 - Octanesulfonic acid sodium salt hydrate and B： methanol, the detection was set at 270 nm, and the flow rate was 0. 4 ml/min. Results In the linear range the correlation coefficients ranged from 0. 999 3 to 0. 999 8. The average recoverie rates were 98. 1% - 100. 2% with corresponding RSD of 0. 17 - 0. 72. The method was successfully applied for determination of vitamin in vitamin beverages and fortified foods. Conclusion This method is proved to be of good speed,specificity,sensitivity and accuracy.