摘要
In order to study the effect of self-made liver preservation solution on liver preservation by comparing with UW solution and HC-A solution, the self-made liver preservation solution （SM） and perfusion solution were prepared under the aseptic conditions. The isolated non-circulated perfusion rat liver model was established. According to the different preservation solutions, the rats were randomly divided into UW group, SM group and HC-A group. The three groups were divided into 6 subgroups according to the preservation duration （n=6 in each group）. The transferase in liver perfusion solution and intercellular adhesion molecule-1 （ICAM-1） and nitric oxide （NO） in liver tissues were determined at 2, 8 and 24 h respectively. The results showed that the levels of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） had no significant difference between SM group and UW group, but significantly lower than in HC-A group. The levels of ICAM-1 and NO were increased simultaneously in SM group and UW group （P〉0.05）, but there was significant difference as compared with HC-A group （P〈0.05）. At the same time point, the level of ICAM-1 was higher in SM group than in UW group, but NO was lower. The preservation effect of SM solution is the same as UW solution, but better than HC-A solution.
In order to study the effect of self-made liver preservation solution on liver preservation by comparing with UW solution and HC-A solution, the self-made liver preservation solution （SM） and perfusion solution were prepared under the aseptic conditions. The isolated non-circulated perfusion rat liver model was established. According to the different preservation solutions, the rats were randomly divided into UW group, SM group and HC-A group. The three groups were divided into 6 subgroups according to the preservation duration （n=6 in each group）. The transferase in liver perfusion solution and intercellular adhesion molecule-1 （ICAM-1） and nitric oxide （NO） in liver tissues were determined at 2, 8 and 24 h respectively. The results showed that the levels of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） had no significant difference between SM group and UW group, but significantly lower than in HC-A group. The levels of ICAM-1 and NO were increased simultaneously in SM group and UW group （P〉0.05）, but there was significant difference as compared with HC-A group （P〈0.05）. At the same time point, the level of ICAM-1 was higher in SM group than in UW group, but NO was lower. The preservation effect of SM solution is the same as UW solution, but better than HC-A solution.
