茶多酚对脑缺血再灌注损伤大鼠 ATP 酶活性和 MDA 含量的影响

Effects of Tea Catechines on Activities of ATPases and MDA Content in Cerebral Ischemia Reperfusion Rats

采用大鼠脑缺血再灌注模型，观察茶多酚对大鼠脑组织Ｎａ＋、Ｋ＋－ＡＴＰ酶、Ｃａ２＋－ＡＴＰ酶、Ｍｇ２＋－ＡＴＰ酶活性和丙二醛（ＭＤＡ）含量的影响。结果表明：（１）茶多酚组Ｎａ＋、Ｋ＋－ＡＴＰ酶、Ｍｇ２＋－ＡＴＰ酶和Ｃａ２＋－ＡＴＰ酶活性高于模型组（Ｐ＜０．０１或Ｐ＜０．０５）。（２）茶多酚组ＭＤＡ含量低于模型组（Ｐ＜０．０５）。（３）模型组Ｎａ＋、Ｋ＋－ＡＴＰ酶活性和ＭＤＡ含量呈负相关，（ｒ＝－０．８８５，Ｐ＜０．０１）；茶多酚组脑组织Ｎａ＋、Ｋ＋－ＡＴＰ酶活性和ＭＤＡ含量也呈负相关，（ｒ＝－０．８４８，Ｐ＜０．０５）。提示茶多酚可通过抗脂质过氧化而实现其保护细胞膜的效应。

To investigate the effects of tea catechine on Na +、K + ATPase、 Ca 2+ ATPase、Mg 2+ ATPase activity and malondialdehyde(MDA) content in brain tissue, a cerebral ischemia/reperfusion model was established The results indicated : (1) Na +、K + ATPase activity of tea catechines group was much higher than that of the model group (P<0 01 or P<0 05)  (2) MDA content of tea catchines group was lower than that of the model group(P<0 05). (3) Both in the tea catechines group and the model group,the Na +、K + ATPase activity was closely related to MDA content(r=0 885,P<0 01 and r=0.848,P<0 05 respectivily).These results showed that the protective effects of tea catechines on ischemia reperfused brain cell might be associated with the inhibition of cell membranes lipid peroxidation