紫堇灵、乙酰紫堇灵及原鸦片碱对小鼠实验性肝损伤的保护作用

PROTECTIVE ACTION OF CORYNOLINE, ACETYLCORYNOLINE AND PROTOPINE AGAINST EXPERIMENTAL LIVER INJURY IN MICE

研究了紫堇灵、乙酰紫堇灵及原鸦片碱对小鼠实验性肝损伤的保护作用及其作用机理。小鼠预先分别ｉｇ紫堇灵、乙酰紫堇灵或原鸦片碱５０及１００ｍｇ·ｋｇ－１２次，对ＣＣｌ４、硫代乙酰胺、扑热息痛所致的小鼠肝损伤均有保护作用，使ＳＧＰＴ显著降低，肝病理损伤程度减轻。此３种成分在体外均能抑制ＣＣｌ４引起的肝微粒体脂质过氧化及ＣＣｌ４转化为ＣＯ。在上述实验中乙酰紫堇灵的作用均强于另外两种成分。另外，此３种成分对肝药酶有先抑制后诱导作用。

Oral administration of two doses of corynoline, acetylcorynoline or protopine at 50 and 100 mg·kg -1 in an interval of 8 to 24 h before ip injection of CCl 4, acetaminophen or thioacetamide significantly impeded the elevation of serum transaminase (SGPT) and liver damage in mice. The three compounds were found to inhibit CCl 4 induced microsomal lipid peroxidation and CCl 4 conversing to carbon monoxide in liver microsomes in vitro . Of these compounds, acetylcorynoline was shown to be more potent than corynoline and protopine. In addition, all the three compounds exhibited biphasic effects on the hepatic cytochrome P450, i.e. inhibition followed by induction, in mice.