川芎嗪注射液对生理状态下大鼠心室组织中硫化氢分泌的影响

Effects of ligustrazine on the secretion of hydrogen sulfide in the ventricular tissues of rats under physiological condition

目的:探讨川芎嗪注射液对大鼠生理状态下心室组织中硫化氢(H2S)分泌的影响。方法:将8只Wistar大鼠左、右心室剪碎后分为4组;正常对照组和川芎嗪低剂量组(1.5μg/ml)、川芎嗪中剂量组(15μg/ml)、川芎嗪高剂量组(150μg/ml),每组n=8。用Krebs液作为孵育液,放入37℃恒温水浴箱中振荡孵育4h后,用放射免疫法测定孵育液中H2S的含量。结果:左心室组织,川芎嗪低剂量组H2S含量(0.17±0.03)和川芎嗪中剂量组H2S含量(0.17±0.03)与对照组H2S含量(0.19±0.03)相比,无统计学意义,川芎嗪高剂量组H2S含量(0.14±0.02)明显低于对照组H2S含量(0.19±0.03)(P<0.01)。右心室组织,川芎嗪低剂量组H2S含量(0.60±0.11)和川芎嗪中剂量组H2S含量(0.53±0.11)与对照组H2S含量(0.64±0.13)相比,无统计学意义,川芎嗪高剂量组H2S含量(0.46±0.09)明显低于对照组H2S含量(0.64±0.13)(P<0.01)。结论:在生理状态下,大剂量川芎嗪具有抑制心室组织释放H2S的作用。

Objective： To explore the effects of ligustrazine on the secretion of hydrogen sulfide in the ventricular tissues of rats under physiological condition. Methods：32 rats were divided into control group, high dose group of ligustrazine（ 150μg/nll）, medium dose group of ligustrazine（15l.μg/ml） and low dose group of ligustrazine （ 1.5μg/ ml） at random. The left and right ventricuiar tissues were incubated in 37℃ thermostatic water bath for 4 hours with Krebs solution as supernatant. After incubation of the ventricular tissues, the sensitive electrode-based method was used to detect the content of H2S in the supernatant. Results： In the left ventricular tissues, there were on differenceof the H2S level betweenthe 1.5μg/ml hgustrazine group（0.17 ± 0.03）, 15μg/ml ligustrazine group（0.17 ± 0.03）and control group. However, compared with control group, the H2S level in hige dose group of ligustrazine decreased obviously（ P 〈 0.01 ）. In the right ventricular tissues, the H2S level in the 1.5μg/ml ligustrazine groups （0.60 ± 0.11 ）and 15μg/ml ligustrazine groups（0.53 ± 0.11 ）had no significantly difference from that in the control group（0.64 ± 0.13） （ P 〉 0.05）, but the H2S level in the 150μg/ml ligustrazine group（0.46 ± 0.09） was significantly lower than that in the control group（0.64 ± 0.13） （ P 〈 0.01 ）. Conclusion： Under physiological condition, high dose of ligustrazine can inhibit the release of hydrogen sulfide in the ventricular tissues of rats.