葛根血中移行成分高效液相色谱分析

HPLC analysis of the constituents of Radix Puerariae absorbed into blood

目的:对常用中药材葛根进行血清药物化学研究,为筛选确定葛根的药效成分奠定基础。方法:在建立葛根高效液相色谱(high performance liquid chromatography,HPLC)指纹图谱的基础上,通过比较葛根甲醇提取物、葛根含药血清和空白血清的HPLC指纹图谱,确定葛根血中移行成分。结果:在葛根含药血清中发现了4个入血成分,其中两个为原型成分(其中一个为葛根素),其余两个可能为原型成分的代谢产物。结论:4个入血成分可能成为葛根的体内直接作用物质,对其进行深入的研究,将有助于阐明葛根的药效物质基础及药效机制。

Objective： To sieve the bioactive constituents of Radix Puerariae, serum pharmacochemistry research was performed. Method： Based on the establishment of HPLC fingerprints of Radix Puerariae, the constituents absorbed into blood were determined by comparing the HPLC fingerprints of the methanol extracts, tested serum samples and blank serum sample. Results： Four compounds absorbed into blood were detected, among which two were original constituents of Radix Puerariae （including puerarin）, the other might be metabolites of the original constituents. Conclusion： These four constituents absorbed into blood were possible bioactive components of Radix Puerariae. Further studies on them will help clarify the bioactive constituents and mechanisms of Radix Puerariae.