黄芪总黄酮对高糖培养下牛视网膜血管周细胞凋亡的影响

Effect of total flavonoids of Astragalus on apoptosis of cultured bovine retinal capillary pericytes under high glucose

目的:探讨黄芪总黄酮对高糖培养下的牛视网膜血管周细胞凋亡的影响。方法:以在体外培养3代融合的牛视网膜血管周细胞为对象,分为正常对照组、高糖组(25 mmol/L)、干预组不同浓度黄芪总黄酮组(0.25、0.5、1.0、2.0 mg/ml),孵育6d,采用TUNEL法检测培养后周细胞凋亡率;硫代巴比妥酸检测培养液丙二醛(MDA)水平;黄嘌呤氧化酶反应系统检测培养液超氧化物歧化酶(SOD)水平。结果:与高糖组比较,黄芪总黄酮0.5、1.0、2.0mg/ml组周细胞MDA含量、SOD活力、MDA含量/SOD活力比值及凋亡率均降低(P<0.01);MDA含量/SOD活力与周细胞凋亡率二者呈正相关(r=0.921,P<0.01);黄芪总黄酮2.0 mg/ml组周细胞氧化应激水平及凋亡率明显低于其他各组(P<0.05)。结论:一定浓度黄芪总黄酮对高糖培养下的牛视网膜血管周细胞凋亡有明显的抑制作用,呈剂量依赖性。

Objective： To study the effects of total flavonoids of Astragalus on apoptosis of bovine retinal capillary pericytes （BRPs） under high glucose. Methods： The third generation of nearly symphysic bovine retinal vessel pericytes cultivated in vitro were divided into normal control group, high glucose group, and Astragalus total flavonoids groups （0.25, 0.5, 1.0, 2.0 mg/ml） at random. After being incubated for 6 days, apoptosis of BRPs were detected by TUNEL method. TBA method was used to detect the eontents of MDA. Xanthine oxidase method was used to detect the SOD activities. Results： Compared with high glucose group, the apoptosis, MDA contents, SOD activity, MDA content/SOD activity of BRPs reduced markedly in total flavonoids of Astragalus groups（0.5, 1.0, 2.0mg/ml） （P 〈 0.01）. The MDA content/SOD activity of BRPs correlated positively with the apoptotic rate of BRPs（ r = 0.921, P 〈 0.01 ）. The oxidative stress levels and apoptosis of BRPs in each total flavonoids group was lower than that of other groups（ P 〈 0.05）. Conclusion： Certain concentration of the total flavonoids of Astragalus can decrease the apoptosis and oxidative stress of BRPs in a dose-dependent manner.