威灵仙多糖的抗氧化活性研究

Study on antioxidant activity of Clematis chinensis Osbeck polysaccharide

目的:研究威灵仙多糖(CCP)的抗氧化活性。方法:①体外测定CCP清除Feton体系产生的羟基自由基(.OH)、邻苯三酚自氧化体系产生的超氧阴离子自由基(O2-.)的活性;②CCP对H2O2诱导的小鼠红细胞自氧化溶血的影响;③CCP对小鼠CCl4急性肝损伤的影响。结果:威灵仙多糖具有清除羟基自由基和超氧阴离子自由基的作用,降低H2O2诱导的红细胞氧化溶血率,可显著提高肝损伤小鼠血清和肝脏中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力,降低丙二醛(MDA)含量及肝脏指数。结论:威灵仙多糖在体内外均具有显著的抗氧化作用,其抗氧化作用与清除氧自由基有关。

Objective： To study the antioxidant activity of Clematis chinensis Osbeek polysaccharide （CCP）. Methods：（1） To assay the scavenging activity of CCP on hydroxyl radical and superoxide anion in vitro. （2） To analyze the effect of CCP on red blood cell autoxidation hemolysis induced by H2O2 by colorimetry. （3） To study the effect of CCP on acute hepatic injury of the mice induced by carbon tetrachloride. Results： CCP could eliminate the hydroxyl free radical and the superoxide anion free radical, reduce red blood cell autoxidation hemolysis induced by H2O2, raise the activities of SOD and GSH-Px in hepatic injury mice＇s serum and liver apparently, reduce the content of MDA and liver index notably. Conclusion： CCP has the significantly action of anti oxidation activity in vitro and in vivo which relates to removing oxygen free radical.