摘要
为快速诊断犬恶丝虫病,建立了犬恶丝虫抗体dot-ELISA检测方法,并与阻断试验、交叉反应试验、重复性试验、琼脂扩散试验和平行对照试验进行了比较。结果显示,制备的犬恶丝虫虫体粗制抗原与犬钩虫、犬弓首蛔虫和犬蠕形螨感染犬的阳性血清无交叉反应,检出犬恶丝虫阳性血清的最高抗体效价为1∶1 024,重复性好;建立的犬恶丝虫病dot-ELISA诊断方法的灵敏度比琼脂扩散试验和美国IDEXX实验室制备的犬恶丝虫病诊断试剂盒分别高2 048倍和48倍。
In order to rapidly diagnose dirofilariasis in dog, a dot-ELISA for detection of antibody against Dirofilaria immitis was established using raw antigen extracted from D. immitis and evaluated by interruption test,cross-reaction test, reproducibility test, agar diffusion test and parallel control test. There was no cross-reaction of the prepared raw antigen with the positive sera from dogs infected respectively with Ancylostoma caninum, Toxocara canis and Demodex canis and the highest titer of the positive serum from dogs infected with D. immitis was 1 ： 1 024 detected by the dot-ELISA,which had good reproducibility. The sensitivity of the dot-ELISA was 2 048 times and 48 times higher than that of the agar diffusion test and Canine Heartworm Antigen Test Kit made by American IDEXX Laboratory,respectively.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2008年第2期106-110,共5页
Chinese Veterinary Science
基金
成都大熊猫繁育研究基金会资助项目(CPF0026)
