大鼠骨髓间充质干细胞同种异体移植治疗急性心肌梗死

Treatment of Acute Myocardial Infarction by Allogeneic Transplantation of Rat Bone Marrow Mesenchymal Stem Cells

目的探讨骨髓间充质干细胞(MSCs)移植治疗急性心肌梗死(AMI)的可行性。方法50只雌性Wistar大鼠随机分为移植组和对照组,各25只。以液氮冷冻法制作大鼠AMI模型,以传代、诱导多次的MSCs作为移植细胞,注入移植组梗死部位,对照组注入等量DMEM液。分别于术前1天、术后1周、术后4周行超声心动图检查,评价心功能。移植组于术后4周处死,移植部位行BrdU免疫组化荧光染色。结果部分移植细胞α-肌动蛋白和肌钙蛋白T免疫组化染色阳性。术后4周,移植组大鼠的心功能优于对照组(P>0.05)。移植部位组织切片可见BrdU标记阳性的细胞核。结论传代、诱导多次的大鼠MSCs体外能分化为心肌样细胞,移植入AMI模型的受损心肌后能存活并有助于改善宿主的心功能。

Objective To investigate the feasibility of acute myocardial infarction （AMI） treated with transplantation of bone marrow mesenchymal stem cells （MSCs）. Methods Fifty female Wistar rats were randomly divided into the transplant group and control group with 25 animals in each group. The AMI animal model was made by liquid nitrogen freezing. Rat MSCs cultured and induced repeatedly was prepared for transplantation and injected into the infarcted parts of the transplant group. The same volume of DMEM solution was injected into the infarcted parts of the control group. Echocardiography was applied one day preoperation, and one week and four weeks postoperation to evaluate cardiac function. The animals of the transplanted group were executed in the fourth week after operation and the tissues of transplant part were examined by BrdU immunofluorescent stain. Results Some transplant cells expressed the cardiac-specific proteins, α-actin and troponin T. The cardiac function of the transplant group had better than that of the control group （ P 〉0.05） in the fourth week postoperation. The BrdU-labeled ceils were found in the histological sections of the transplant parts. Conclusion Rat MSCs cultured generation by generation and repeatedly induced by 5-aza in vitro can differentiate into cardiomyocyte-like cells, which if transplanted into the rat infarcted cardiac muscles will survive and help to improve the host＇s cardiac function.