中药合剂治疗佐剂性关节炎大鼠的拆方实验研究

Experimental Research on Adjuvant Arthritis Treated with Different Combinative Herbal Mixture

目的观察临床治疗类风湿性关节炎使用频率最高的解表药(麻黄)、祛风湿药(川乌)、活血药(当归)及其复方合剂对佐剂性关节炎大鼠的影响,并研究其配伍规律。方法把有相似作用的药物配成常用药对,制成麻桂合剂、制川草乌合剂、归芎合剂、复方合剂、低剂量复方合剂,并以正常组、造模组、雷公藤组做对比。用ELISA法和分光光度计测量足爪肿瘤坏死因子α(TNF-α)和前列腺素E2(PGE2)的水平,测定免疫器官的脏器重量指数,分析不同药物组对免疫功能的影响。结果造模组足爪PGE2、TNF-α水平明显高于正常组(P<0.01),复方组与雷公藤组能抑制足爪PGE2水平(P<0.05),复方组、祛风湿组、雷公藤组都能明显抑制关节滑膜TNF-α水平(P<0.01),低剂量复方组也能抑制关节滑膜TNF-α水平(P<0.05),造模组脾与胸腺的脏器指数有升高趋势(脾脏指数P<0.05)。结论中药复方配伍能抑制大鼠足爪PGE2、TNF-α水平,制川草乌合剂发挥主要作用。

Objective To observe the effect of Chinese herbs most used in rheumatoid arthritis such as diaphoretics （ephedra）, wind-expelling herbs （monkshood）, blood activating drugs （angelica） and compound mixture in treating adjuvant arthritis, and study the compatibility law. Methods AA rats in groups were treated with ephedra-cinnamon twig mixture, monkshood-aconitum mixture, angelicachuanxiong mixture, herbs compound mixture, low dose herbs compound mixture which is composed of above-mentioned herbs and semblable function herbs. Compare with normal group, model group, tripterygium wilfordii hook （TWH） group, TNF-α and PGE2 level of hindpaw were measured by ELISA and spectrophotometer. Weight index of immune organs were measured to study the effect of different functional ingredient groups and complex on immune function. Result The hinderpaw PGE2 level and TNF-α level of model group was higher than normal group （P〈0.01）. Herbs compound mixture and TWH could inhibit the hinderpaw PGE2 level （P〈0.05）. Herbs compound mixture, wind-expelling herbs and TWH could obviously inhibit the hinderpaw TNF-α level （P〈0.01）. Low dose herbs compound mixture could also inhibit the hinderpaw TNF-α level （P〈0.05）. The weight index of spleen and thymus of the model group showed a tendency to increase （spleen weight index P〈0.05）. Conclusion The Chinese herbs compound can inhibit PGE2 and TNF-α level of hindpaw in rats, wind-expelling herbs play primary roles in the compound mixture.