强肌健力口服液含药血清对大鼠骨髓间充质干细胞体外增殖的影响

Effects of Serum Containing Qiangjijianli Oral Liquid on In-Vitro Proliferation of Rat Mesenchymal Stem Cells

目的观察强肌健力口服液含药血清对大鼠骨髓间充质干细胞(MSCs)体外增殖的影响。方法使用密度梯度离心法分离大鼠MSCs,5-溴脱氧嘧啶(Brdu)和CD44、CD54双重免疫组化鉴定,传3代后的MSCs分为强肌健力口服液含药血清组和空白血清对照组,四甲基偶氮唑蓝(MTT)法检测2组MSCs的生长情况。结果强肌健力口服液含药血清组以浓度依赖方式促进MSCs的增殖活力,与相应浓度的空白血清对照组比较差异有显著性意义(P<0.05,P<0.01)。结论补脾法(强肌健力口服液含药血清)能促进干细胞的生长。

Objective To observe the effect of serum containing Qiangjijianli oral liquid in-vitro proliferation of rat mesenchymai stem cells （MSCs）. Methods Rat mesenchymai stem cells dissociated from the bone marrow by density gradient method were cultured. MSCs were identified by marking of bromodeoxyuridine （Brdu） and staining of CD44, CD45. Experimental group was cultivated with serum containing Qiangjijianli oral liquid and control group with blank serum. Optical absorption value of MSCs was stained by methyl thiazolyl tetrazolium （MTT）. Results Serum containing Qiangjijianli oral liquid at different concentrations could promote the proliferation of MSCs, the difference being significant in comparison with the control group （P 〈0.05 or P 〈0.01）. Conclusion Invigorating the spleen （serum containing Qiangjijianli oral liquid） can enhance the proliferation of MSCs.