反相高效液相色谱法测定肤疾洗剂中苦参碱的含量

RP-HPLC Determination of Matrine in Fuji Lotion

目的建立肤疾洗剂中苦参碱的含量测定方法。方法采用反相高效液相色谱法,InertsilNH2色谱柱(250mm×4.6mm,5μm),以乙腈-无水乙醇-3%磷酸溶液(83∶8.5∶8.5)为流动相,流速1.0mL/min,检测波长为220nm。结果苦参碱在0.039～1.560μg范围内线性关系良好(r=0.9997),平均回收率为97.8%,RSD=1.1%。结论本法简便、快速、准确,可用于该制剂的质量控制。

Objective To establish a method for determination of matrine in Fuji Lotion. Methods An Inertsil NH2 column （250 mm× 4.6 mm, 5 μm） was used with acetonitrile-ethanol absolute-3% phosphoric acid （83 ： 8.5 ： 8.5） as mobile phase, the flow rate was 1.0 mL/min, and the UV detection wavelength was set at 220 nm. Results The calibration curve of matrine was linear in range of 0.039-1.560 lag, r =0.9997. The average recovery was 97.8% with RSD of 1.1% （n =6）. Conclusion The method is simple, quick and accurate. It can be used for the quality control of Fuji Lotion.