去甲肾上腺素预处理早时相线粒体跨膜电位的变化

Change of Transmembrane Potential During the Early Preconditioning Phase of Noradrenaline's Treatment

目的研究去甲肾上腺素(NA)预处理早时相心肌细胞线粒体跨膜电位的变化,为探讨预适应早时相作用机制提供依据。方法原代培养乳鼠心肌细胞,随机分为3组进行干预处理。Ⅰ组是对照组(control group,CON),Ⅱ组是缺氧处理对照组(hypoxia group,HP),Ⅲ组是去甲肾上腺素预处理组(noradrenaline preconditioning group,NAPC)。Ⅰ组不给任何药物干预,也不进行缺氧处理,培养40min;Ⅱ组不给任何药物干预,直接进行缺氧处理40min;Ⅲ组预先给予终浓度为0.2μg/ml去甲肾上腺素孵育10min后,再进行缺氧处理40min,各组用流式细胞仪检测罗丹明123(Rhodamine123)标记的线粒体的荧光强度。结果3组的荧光强度值通过方差分析比较(F=461.3,P<0.05),HP组与NAPC组的荧光强度比CON组明显降低(6.24±0.13)%,(6.55±0.17)%vs.(8.32±0.19)%,P<0.05,表明HP组与NAPC组心肌细胞线粒体的跨膜电位明显降低,说明细胞能量代谢能力下降;NAPC组荧光强度比HP组高(6.55±0.17)%vs.(6.24±0.13)%,P<0.05,表明NAPC组心肌细胞线粒体的跨膜电位较高,说明NA预处理过的细胞能量代谢能力较强。结论NAPC组的心肌细胞线粒体的荧光强度较HP组明显升高,表明去甲肾上腺素预处理早时相跨膜电位降较高,NA触发了细胞保护效应,使心肌细胞对损伤性刺激的耐受力增强。

Objective To investigate the change of transmembrane potential during Noradrenaline ＇ s early preconditioning phase and probe the mechanism of action. Methods Primary cuhure myocardial cell of neonate rat were divided into three groups at random. Group Ⅰis control group, Group Ⅱ is treatment group by hypoxia, Group Ⅲis treatment group by NA. Group Ⅰ didn＇t give any drug to culture for 40minutes; .Group Ⅱtreated for 40minutes by hypoxia, Group Ⅲexposed to Noradrenaline （0.2μ g/ ml） for 10 minutes then treated with hypoxia for 40 minutes. The fluorescence intensity of rhodamine-123 marked mitochondria were detected by flow cytometry. Results The fluorescence intensity of three Group were test by analysis of variance （ F = 461.3, P 〈 0. 05 ） The fluorescence intensity of GroupHPand Group NAPC is lower than Group CON [（6.24±0.13）%, （6.55 ±0.17）% vs. （8.32±0.19）%, P〈0.05）]. The transmembrane potential of Group HP and Group NAPC is lower than Group CON which indicated the energy synthesis is not enough. The fluorescence intensity of Group NAPC is higher than Group HP. The transmembrane potential of Group NAPC is higher than Group HP which indicated the energy synthesis of Group HP is not enough. Conclusions The fluorescence intensity of Group NAPC is higher than Group HP which indicate the transmembrane potential of Group NAPC is higher during the early preconditioning of NA. NA can strengthen tolerance of myocardial cell.