昆明地区汉族人群9个Y-STR_S基因座单倍型分析及其旁系亲子鉴定应用

Haplotype Analysis of 9 STR_S Loci on Human Y Chromosome in Han Ethnic Group of Kunming City

目的调查昆明地区汉族人群Y染色体DYS390、DYS393、DYS437、DYS438、DYS439、DYS447、DYS448、DYS460、H4,9个基因座的遗传多态性,获取9个基因座的单倍型数据。方法应用苯酚∶氯仿及Chelex-100萃取法提取DNA,采用STR-PCR、非变性聚丙烯酰胺凝胶电泳、银染技术和377测序技术,对昆明地区汉族群体216例男性个体就上述9个基因座进行单倍型分析。结果216例样本中9个基因座得到216种单倍型,每种单倍型的频率均为0.0046,GD=100005934437,S.E.=125×10-9(GD,genediversity,基因多样性)。结论昆明汉族9个Y-STRs基因座具有高度多态性,在法医学特殊亲权鉴定中具有良好的应用前景。

Objective To study the haplotype diversity of DYS390,DYS393,DYS437,DYS438,DYS439,DYS447,DYS448,DYS460, H4 loci in 216 unrelated individuals in Han ethnic group of Kunming city , Yunnan province. Methods The DNA were extracted with Chloroform,phenol methods or Chelex - 100 method. STR - PCR , Non - denaturing polyacylaminde gel electrophoresis and Sliver staining and ABI377 sequencer were carried out for study. 216 undividuals were collected from Han ethnic group in Kunming city. Results 216 types of haplotype were detected from 9 STRS. Every haplotype frequence was detected at 0. 0046, GD = 1. 00005934437, The lowest arrangement of the repeat units was detected at 0. 0046. S E = 1.25 ×10^-9 o Conclution The 9 Y - STRs loci of Kunming people are highly polymorphic and are powerful means in determination of identify and paternity for forensic practice.