摘要
目的在大鼠离体心脏缺血再灌注模型上,观察纳洛酮对大鼠心肌氧自由基代谢的影响,探讨纳洛酮对心脏作用的可能机制。方法建立改良的Langendorff离体大鼠心脏灌注模型,缺血40min,再恢复常速灌注30min,造成心肌缺血再灌注损伤。利用该模型观察给予不同浓度的纳洛酮后大鼠心肌SOD活性及MDA含量的变化。结果模型组与对照组比较,心肌MDA含量增加,SOD活力降低;给予两种不同浓度的纳洛酮分别灌注缺血的心脏后,与对照组比较,纳洛酮16mg/L和32mg/L可减少心肌MDA生成量,并提高SOD活力。结论纳洛酮对大鼠再灌注损伤心脏的具有保护作用,其机制与抗氧化作用有关。
Objective TO study the effects of Naloxone in free radial on myocardial ischemia - reperfusion injury. Methords A langendorff rats model were builded, then isolated rat hearts were subjected to ischemia for 40 min and reperfusion for 30 min. myocardial levels f malondialdehyde（MDA） and the activity of superoxide dismutase （SOD） were detected . Results Myocardial enzyme （LDH） and MDA in model group was significantly increase than control group; the activity of SOD was decreased（P 〈 0.01 ）. In the NAL group the levels of MDA and LDH reduced, the activity of SOD significantly higher than model group （ P 〈 0.05 ）. Conclusions the results suggested that NAL had protective effects on alleviated reperfusion injury after ischemia, Which might be associated with enhancing the ability of antroxygen free radical.
出处
《医学研究杂志》
2008年第2期56-57,共2页
Journal of Medical Research
基金
Effectso
