人支气管上皮细胞体外不同培养基培养效果比较

Comparison of different media of human bronchial epithelial cells

目的:比较胎牛血清培养基,小牛血清培养基对经纤维支气管镜(纤支镜)刷检人支气管上皮细胞培养存活率的差异。方法:采用不同细胞培养液,对经纤支镜刷检获得的人支气管上皮细胞进行原代培养,并通过倒置相差显微镜观察原代培养细胞,比较细胞的存活率。结果:胎牛血清培养基细胞成活率比其他培养基高。结论:胎牛血清培养液可提供较为满意的生长条件。

Objective：To compare the difference live rate of media for culturing human bronchial epithelial cells obtained by bronchofiberscopic brushing between fetal bovine serum （FBS） and newborn calf serum （NCS） . Methods： Human bronchial epithelial cells obtained by bronchofiberscopic brushing were cultured in fetal bovine serum （FBS） or newborn calf serum （NCS） . And the cells were observed by microscope and scanning electronmicroscope. Then the live rate was compared between the two serums. Results： Cells cultured in FBS could be obtained in high live rate. Conclusion：FBS could provide a good live condition for human bronchial epithelial cells.