基因枪介导获得转植酸酶基因水稻研究

Transformation of Phytase Gene into Rice by Particle Bombardment

植酸酶是一类能促进植酸及其盐类水解成肌醇和磷酸的酶的总称.它能提高饲料和食品中磷的利用率,减轻粪便中磷排放所造成的环境污染,清除植酸与金属离子的螯合作用,改善微量元素的吸收和利用.本研究将马铃薯的胞外分泌信号肽(Psec)和大肠杆菌的植酸酶基因(appA2)结合组成融合基因导入粳稻台北309中.PCR检测和Southern杂交表明,植酸酶基因和分泌信号肽序列已经整合到水稻的基因组中.无机磷含量分析表明,含目的基因的转基因水稻种子及茎叶中的无机磷含量均较未转化植株有显著的提高.

Phytase,catalyzing the hydrolysis of phytate to myo-inositol and inorganic phosphate, can improve the bioavaibility of phytate phosphorus in human food and animal feedstuff, reduce phosphorus pollution of animal waste, eliminate chelation between phytate and metallic ion, and increase the absorption and utilization of nutritious substance. In this study, potato extracellular secrete signal sequence （Psec） and phytase gene （appA2 ） cloned from Escherichia coli were transformed into genome of rice variety Taipei 309. The results of PCR detection and Southern blot showed that the appA2 and Psec were inserted into rice genome. The analysis data of inorganic phosphorus indicated that the inorganic phosphorus content in the mature seeds, leaves and stem of the transgenic rice were higher than that of the control. Fig 6, Tab 1, Ref 14