脑胶质瘤survivin表达及其反义核酸对U251细胞的作用

The survivin expression in brain glioma tissues and effection of survivin antisense oligodexynucleotides on U251 cells

目的：观察脑胶质瘤患者脑组织生存素（survivin）的表达及其反义寡核苷酸（survivin ASODN）对脑胶质瘤U251细胞生长和凋亡的影响。方法：RT-PCR法检测脑胶质瘤患者脑组织survivin mRNA表达,脂质体介导下,分别以100、300、500 nmol/Lsurvivin ASODN作用于脑胶质瘤U251细胞48 h后,观察细胞形态学,流式细胞术检测细胞凋亡。结果：脑胶质瘤患者脑组织有survivin mRNA表达,阳性率为76.7%。不同浓度survivin ASODN转染48 h后,细胞生长抑制率分别为（52.24±6.59）%、（36.14±4.01）%和（24.14±5.57）%,凋亡指数分别为（19.46±3.85）%、（38.6±1.85）%和（55.34±3.16）%,与对照组及不同浓度组间比较差异有统计学意义（P〈0.05-P〈0.01）。结论：脑胶质瘤患者脑组织有survivin mRNA表达,survivinASODN以剂量依赖方式抑制脑胶质瘤U251细胞生长,诱导细胞凋亡。

Objective： To observe the expression of survivin in brain glioma tissues and effects of survivin antisense oligodexynucleodides （ ASODN ） on the proliferation and apoptosis of U251 cells. Methods： RT-PCR method was performed for detecting survivin mRNA expression in specimens. The glioma cells U251 transferred with survivin ASODN for 48 h, the proliferation and apoptosis were measured by flow cytometry（ FCM ） and cell morphology. Results：Survivin mRNA was expressed in brain glioma tissues, and the positive rate was 76.7%. When U251 cells were treated with different concentration of ASODN at 100 nmol/L,300 nmol/L, and 500 nmol/L,the apotosis rates were （ 19.46 ± 3.85 ）% , （38.60 ± 1.85 ）% , and （55.34 ± 3. 16）%, respectively; and the proliferation index（PI） were （ 52.24 ± 6.59 ） % , （ 36.14 ± 4.01 ） % , and （ 24.14 ± 5.57 ） % , respectively, the difference was distinct compared with control and groups of different concentration （ P 〈 0.05 - P 〈 0.01 ）. Conclusions： Survivin mRNA was expressed in the brain glioma tissues, survivin ASODN efficiently inhibits the proliferation and induce apotosis of U251 cells in a dose- dependent manner.