木霉菌ECT-01-2对人参锈腐病菌的拮抗作用

Antagonism of Trichoderma sp.ECT-01-2 against Ginseng Rust Rot Pathogens

以木霉菌株ECT-01(Trichoderma sp.)为出发菌株,孢子经紫外线照射处理后获得1株高拮抗活性的突变体ECT-01-2。通过对峙培养与发酵液处理,研究突变体ECT-01-2对人参锈腐菌的拮抗作用。结果表明,ECT-01-2对人参锈腐菌的抑制率高达83.68%。发酵液对人参锈腐菌丝和孢子都有一定的抑制作用,初步明确了ECT-01-2对人参锈腐菌的拮抗机理以竞争、重寄生、抗生作用及溶菌作用为主。

A mutant strain ECT- 01 -2 with increased antagonistic activity was produced by treating the original spore of ECT- 01（Trichoderma sp. ） with UV. The antagonism of this induced mutant strain against Cylindrocarpon destructan was studied by means of antagonistic experiments and treating pathogen with its fermentative liquid. The result showed that the inhibition frequency of ECT- 01 -2 to Ginseng rust rot pathogens increased by 83.68% ,and the fermentative liquid could inhibit both the mycelial growth and spore germination of Cylindrocarpon destructan. The mechanism of this strain against Cylindrocarpon destructan mainly included the competition, mycoparasitism, antibody-secretion and cell lysis.