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分子生物学技术在产甲烷古菌研究中的应用 被引量：13

Application of Molecular Biology Technology in Methanoarchaea Research
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摘要 鉴于产甲烷古菌在环境和能源两大方面的特殊作用,对它的研究一直是微生物学研究的焦点之一。现代分子生物学技术的发展与应用,进一步推动了对产甲烷古菌的研究。本文将综述产甲烷古菌基因克隆研究进展以及几项有代表性的分子生物学技术在产甲烷古菌研究中的应用。 In view of the fact that the Methanoarchaea has the special functions in the environment and energy aspect, its research has been one of focal points of microbiology research. The modern molecular biology technology＇s development and the applications further impelled Methanoarchaea research. This article summarized research development of gene cloning of Methanoarchaea, as well as several representative molecular biology techniques applied in the Methanoarchaea research
作者 王刘阳 尹小波 胡国全
机构地区 农业部沼气科学研究所
出处 《中国沼气》 2008年第1期19-24,共6页 China Biogas
基金 国家高技术研究发展计划(863计划)(2006AA10Z420) 四川省科技攻关项目(2006207-004-02) 四川省应用基础研究课题(04JY029-03) 西藏自治区重点科技招标项目
关键词 产甲烷古菌 基因克隆 DGGE TGGE FISH Methanoarchaea gene cloning denaturing gradient gel electrophoresis （DGGE） temperature gradient gel electrophoresis（TGGE） florescence in situ hybridization（FISH）
分类号 Q939.9 [生物学—微生物学] S216.4 [农业科学—农业机械化工程]
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参考文献49

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二级参考文献57

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中国沼气
2008年 第1期

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