适用于糖尿病患者皮鞋鞋材的抗菌防霉复配剂的筛选

Selection of Combined Antimicrobial Agents for Diabetic Shoe Leather Materials

利用具有不同抗菌机理的五种抗菌防霉剂,即4-(2-苯并噻唑基亚氨甲基)苯氧乙酸锌(A)、尼泊金丁酯(B)、山梨酸(C)、百菌清(D)、异噻唑啉酮(E)之间的协同和加合作用,成功开发出了用于糖尿病患者皮鞋鞋材的复配型抗菌防霉剂。首先,以糖尿病患者鞋内分离出来的代表性细菌、酵母菌和霉菌为供试菌种,采用滤纸条交叉放药法对五种抗菌组分进行了复配,结果表明,去除对菌种有拮抗作用的复配组分,有效的复配组分为:A和B、A和E、B和C、B和E、C和E。然后,以等浓度的混合菌为供试菌种,对各种有效组分进行不同比例的复配,并测定了复配后的抑菌圈直径,结果显示,优化复配比例为:A∶B=3∶7、2∶8、1∶9;A∶E=6∶4、4∶6、2∶8;B∶E=5∶5、2∶8;C∶E=3∶7、2∶8。用这些优化复配组分可以有效的抵御糖尿病患者鞋内的病原微生物,这就为它们在糖尿病患者鞋材上的应用提供了参考。

Five antimicrobial components, viz. zinc （∏） complex of 4-E（2-benzothiazolylimino） methyl] phenoxyacetic acid （A）, butyl-p-hydroxybenzonate （B）, sorbic acid（C）, 2,4,5,6-tetrachloro-1,a-benzenedinitrile（TBN, D） and isothiazolinone（E）, which have different antimicrobial modes of action, were chosen to develop effective combined antimicrobial agents used for diabetic shoe leather materials. Firstly, the types of interaction among the five compounds were evaluated by the crossed filter paper strip method, with representative bacteria, yeasts and moulds isolated from the diabetic shoe leather materials as the experimental microorganisms. Results showed that the effective combined agents were A/B, A/E, B/C, B/E and C/E. Then, the diameters of inhi bition zones of the combinations with different concentrations, against the mixed microorganisms isolated from the diabetic shoe leather, were determined to attain the excellent combination rati os. Results showed that the excellent combination ratips were as follows： A ： B=3 = 7,2 ： 8,1 ： 9;A ： E=6 ： 4,4 ： 6,2 ： 8;B ： E=5 ： 5,2 ： 8;C ： E=3 ： 7,2 ： 8. These combinations can block the growth of pathogenic microorganisms in the diabetic shoe and contribute their application to diabetic shoe leather materials.