HPLC法测定川射干中鸢尾黄酮苷的含量

Determination of Tectoridin in Rhizoma Iridis Tectori by HPLC

目的：建立以高效液相色谱法测定川射干中鸢尾黄酮苷含量的方法。方法：色谱柱为Diamond ODS C18（150mm×4．6mm，5μm），流动相为甲醇-0．5％醋酸溶液（3：7），检测波长为265nm，流速为0．8mL·min^-1。结果：鸢尾黄酮苷进样量在0．0792～0．3960μg范围内与峰面积积分值呈良好线性关系；平均回收率为99．80％，RSD：1．09％（n=6）。结论：本方法灵敏、简便、准确，可用于川射干的质量控制。

OBJECTIVE： To determine the content of Tectoridin in Rhizoma Iridis Tectori by HPLC. METHODS： The chromatographic separation was performed on Diamond ODS Cla column（150 mm × 4.6 mm, 5 μm） with mobile phase consisted of MeOH- 0.5% acetic acid（3 ： 7） at a flow rate of 0.8 mL · min ^-1. The detection wavelength was set at 265 nm .RESULTS： Good linear relationship was achieved over the concentration range of 0.079 2～0.396 0 big for tectoridin with an average recovery of 99.80% （RSD = 1.09%, n = 6） . CONCLUSION： The method is sensitive, simple and accurate, and it can be used for the quality control of Rhizoma Iridis Tectori.