注射用核糖核酸对小鼠免疫功能的影响

Effects of ribonucleic acid II on murine immune functions

目的:探讨注射用核糖核酸对免疫系统功能的影响。方法:以小鼠为动物模型,观察不同剂量(0.2、1、5、25mg/kg)注射用核糖核酸对小鼠体重、免疫器官相对重量、细胞免疫功能、体液免疫功能、小鼠迟发性变态反应及巨细胞吞噬功能的影响。结果:与对照组相比,注射用核糖核酸各剂量对小鼠体重及脾脏和胸腺相对重量没有明显影响。注射用核糖核酸4个剂量组小鼠DTH足增加厚度分别为0.34±0.18,0.47±0.14,0.53±0.17,0.52±0.20mm,而对照组为0.24±0.09mm,有显著差异;ConA刺激的脾淋巴细胞增殖试验中注射用核糖核酸4个剂量组A增加值分别为0.44±0.12,0.52±0.14,0.56±0.09和0.60±0.12,明显高于对照组0.34±0.15;Iglu-PFC数目明显增加,每5×106个脾细胞中PFC数目分别为:3444±842,4652±1114,5656±1569,6560±1812,对照组仅为2335±442;3个剂量组明显增强小鼠巨嗜细胞吞噬功能,吞噬率(%)分别为0.36±0.07,0.39±0.07,0.46±0.05,对照组仅为0.28±0.06。结论:核糖核酸具有增强小鼠免疫功能的作用。

Objective-To study the effects of ribonucleic acid Ⅱ on murine immune functions, Methods： Kunming mice （6 -8 weeks old） were administered with ribonucleic acid Ⅱat dosages of 0, 2,1,5,25 mg/kg respectively. Spleen and thymus were weighted and cell-mediated immune functions,humoral immune functions, delayed type hypersensitivity reaction and macrophage function assays were performed by conventional methods. Results： In comparison with control group （0.24 ± 0.09 mm）, 0.2,1,5,25 mg/kg ribonucleic acid I] increased the foot pad swelling to 0.34 ± 0.18, 0.47 ± 0.14, 0.53 ± 0.17, 0.52 ± 0.20 mm respectively. Lymphocyte proliferation capacity was also enhanced. IgM - PFC number increased significantly to 3 444 ± 842, 4 652 ± 1 114, 5 656 ± 1 569, and 6 560 ± 1 812 per 5×10^6 spleen cell respectively at the dose of 0.2,1,5,25 mg/kg, significantly higher than that of control. Also, phagocytosis capacity was significantly enhanced. Conclusion：Ribonucleic Acid Ⅱcould improve the immune functions in mice.