复方二仙汤中盐酸小檗碱及总生物碱的含量测定

Determination of berberine hydrochloride and total alkaloids in compound Er-xian decoction

目的：建立复方二仙汤中盐酸小檗碱和总生物碱的含量测定方法。方法：小檗碱的含量测定应用Hypersil—BDSCi8色谱柱，甲醇-0．02moL／LNaH2P04-0．1moL／L三乙胺（40：60：2）为流动相，检测波长为345nm：采用可见分光光度法于420nm波长处测定总生物碱的含量。结果：盐酸小檗碱的线性范围为0．51—10．10μg／mL，r=0．9999，回收率100．07％，RSD为0．4％；总生物碱线性范围为8．0—24．0μg/mL，r=0．9998，回收率100．46％，RSD为0．58％。结论：该法操作简便、快速且结果准确，可用于该复方的质量控制。

Objective： To establish a quantitative analysis method for berberine hydrochloride and total alkaloids in compound Erxian decoction, Methods： Berberine hydrochloride were determined by HPLC. Using Hypersil-BDS C18（4.6×200 mm） column, methanol-0.02 mol/L sodium dihydrogen phosphate-0.1 mol/L triethylamine （40：60：2） as the mobile phase. The detection wavelength was 345 nm, Total alkaloids were determined at 420 nm by visible spectrometry. Results： The linearity range of berberine hydrochloride was 0.51 - 10.10μg/mL, r = 0.999 9, and average recovery was 100.07% ,RSD O. 4%. The linearity range of total alkaloids was 8.0-24.0μg/mL, r = 0.999 8, and average recovery was 100.46%, RSD O. 58%. Conclusions ： The method is simple, quick and accurate, It can be used to control the quality of the decoction preparation.