摘要
目的研究强脉冲光（IPL）照射对人皮肤成纤维细胞Ⅰ型前胶原mRNA表达水平的影响。方法用强脉冲光对目标皮肤进行连续照射，在照射前及照射后取照射部位皮肤组织，应用实时荧光定量PCR法测定各时间点成纤维细胞Ⅰ型前胶原mRNA的表达水平。结果连续的强脉冲光照射可以显著提高Ⅰ型前胶原mRNA的表达水平。照射2，3次后皮肤成纤维细胞Ⅰ型前胶原mRNA表达[（7．9±1．7）×10^-4、（11．1±2．4）×10^-4]明显高于照射前[（2．1±0．7）×10^-4]，P〈0．05。结论强脉冲光可促进皮肤成纤维细胞Ⅰ型前胶原mRNA的表达，证实了强脉冲光的除皱作用。
Objective To investigate the effects of intense pulse light （IPL） on the mRNA expression of type Ⅰ procollagen in human fibroblasts. Methods Four healthy female subjects aged 19 - 41, underwent IPL on the back skin 3 times with an interval of 2 weeks. Before the IPL and after 1,2, and 3 times of treatment samples of back skin were obtained to undergo real time quantitative PCR to detect the mRNA expression of type Ⅰ procollagen. Results The mRNA expression level of type Ⅰ procollagen after one time of IPL treatment was [ （4.7 ± 1.0）×10^-4] , not significantly different from that before treatment [ （2.1 ±0.7） ×10^-4, P =0. 100]. The mRNA expression levels of type Ⅰ procollagen after treatment for 2 times and 3 -time were （7.9 ± 1.7）× 10^-4 and （11.1 ±2.4） × 10^-4 respectively, both significantly higher than that before treatment （ both P 〈 0.05 ）. Conclusion Promoting the mRNA transcription of type Ⅰ procollagen in dermal fibroblasts, IPL therapy is effective in dermal remodeling and wrinkle removing.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2008年第6期398-399,共2页
National Medical Journal of China
