摘要
目的了解临床分离阴沟肠杆菌中新型氨基糖苷类乙酰转移酶基因[aac(6’)---Ib-cr基因]的分布。方法PCR检测aac(6’)-Ib-cr基因并将阳性扩增产物进行测序分析;纸片扩散法测定aac(6’)-Ib-cr基因阳性菌株对13种抗菌药物的敏感性;改良三维试验检测高产AmpC酶和ESBLs;ERIC-PCR法进行aac(6’)-Ib-cr基因阳性菌株同源性检测。结果81株阴沟肠杆菌中,3株aac(6’)-Ib-cr基因阳性(3.7%);aac(6’)-Ib-cr基因阳性菌株对氟喹诺酮类,氨基糖苷类,氯霉素,氨苄西林,头孢西丁,第2、3代头孢菌素等显示多重耐药2株产ESBLs和AmpC,1株产ESBLs;ERIC-PCR电泳图谱型显示,3株aac(6’)-Ib.cr基因阳性细菌均不属于同一型别。结论临床分离阴沟肠杆菌中存在aac(6’)-Ib-cr基因阳性菌株,为多重耐药、非克隆传播菌株,临床应加强检测和监测。
Objective To investigate the prevalence of aac(6')-Ib-cr gene in Enterobacter cloacae isolates. Methods PCR and sequencing were performed on 81 strains of Enterobacter cloacae isolated clinically in Anhui province to identify aac (6')-IB-cr gene. Disk diffusion method was used to test the susceptibility of the Enterobacter cloacae isolates with aac (6')-Ib-cr gene to fluoroquinolones, aminoglycosides, and other antimicrobial agents. AmpC and extended-spectrum β-lactamases (ESBLs) were detected by modified three-dimensional extract test, and the molecular typing was analyzed by ERIC-PCR. Results aac (6')-Ib-cr gene was identified in 3 (3.7 % ) of the 81 Enterobacter cloacae isolates with different ERIC-PCR patterns. The isolates with aac (6')-Ib-cr gene were resistant to fluoroquinolones, aminoglycosides, chloramphenicol, ampicillin, cefoxitin, and second and third generation cephalosporins. Two of the 3 isolates produced AmpC and ESBLs, and 1 isolate produced only ESBLs. Conclusion aac (6')-Ib-cr gene is prevalent in Enterobacter cloacae isolates with resistance to most of antimicrobial agents and no clone spread in found in them.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2008年第6期422-424,共3页
National Medical Journal of China
基金
中国博士后科学基金资助项目(20060400202)
安徽省自然科学基金资助项目(050430804)
安徽省高等学校省级自然科学研究基金资助项目(KJ2007B145)
关键词
肠杆菌
阴沟
氨基糖苷类
抗药性
Enterobacter cloacae
Aminoglycosides
Drug resistance